Coculture of Bovine Demiembryos Prior to Freezing

Investigating Different Methods of Closed Shell Pistachios Splitting and Effects of Freezing Prior to Drying on Shell Splitting Percentage

In this study, different methods for shell splitting and the effect of freezing prior to drying on shell splitting percentage of pistachio were investigated. A completely randomized design was used to investigate the effects of different freezing temperatures (0, -6, -12 and -18°C), different drying temperatures (80, 90 and 100°C) and different cultivars (Akbari and Kalehghouchi) on shell split...

Percutaneous freezing of sensory nerves prior to total knee arthroplasty.

BACKGROUND Total knee arthroplasty (TKA) is a common procedure resulting in significant post-operative pain. Percutaneous cryoneurolysis targeting the infrapatellar branch of the saphenous nerve and anterior femoral cutaneous nerve could relieve post-operative knee pain by temporarily blocking sensory nerve conduction. METHODS A retrospective chart review of 100 patients who underwent TKA was...

“Short Paper” Application of Gamma Irradiation to Increase the Motility and Viability of Bovine Sperm after Freezing and Thawing

This study was conducted to determine the suitable dose of gamma rays for increasing sperm motility and viability after thawing and to assess its effects on malondialdehyde concentration and the rate of sperm DNA strand breaks. Sperm samples in liquid nitrogen were irradiated at doses of zero, 0.1, 0.3, 0.5, 0.7 and 0.9 Gy. The sperm quality and quantity parameters were evaluated before and aft...

Freezing Bovine Sperm with Egg Yolk

Bull spermatozoa were pellet frozen on dry ice in a TES buffer containing combinations of egg yolk and glycerol to determine which component of an extender afforded cryoprotection to the spermatozoa. Neither buffer nor buffer plus glycerol protected sperm cell motility in the absence of egg yolk, while sperm cells frozen in egg yolk buffer in the absence of glycerol had 24% postthaw motility (P...

A Simple Method for Freezing Bovine Embryos